Table 2.

Values of the fluorescence quantum yield and excited-state lifetime of measured photoswitchable proteins in comparison with the literature values.

Protein	${\mathrm{\Phi }}_{meas}^{100\%}$	${\mathrm{\Phi }}_{meas}^{50\%}$	${\mathrm{\Phi }}_{lit}^{}$	${\tau }_{meas}^{100\%}\left(ns\right)$	${\tau }_{meas}^{50\%}\left(ns\right)$	${\tau }_{meas}^{10\%}\left(ns\right)$	${\tau }_{lit}^{}\left(ns\right)$
Dreiklang	0.47 ± 0.01	0.45 ± 0.01	0.4134	2.9	2.9	2.9	---
Dronpa	0.69 ± 0.01	0.71 ± 0.01	0.6831, 0.8532	3.3	3.3	3.3	---
DronpaM159T	0.22 ± 0.02	0.21 ± 0.01	0.2351	1.2	1.2	1.2	0.6–0.952
rsCherry	0.22 ± 0.02	0.20 ± 0.01	0.0253, 0.00953	1.2	1.2	1.2	---
rsCherryRev	0.08 ± 0.03	0.10 ± 0.01	0.00541,53, 0.00241, 0.000353	0.7	0.7	0.8	---
rsEGFP	0.40 ± 0.01	0.40 ± 0.01	0.3654	1.8	1.8	1.8	---
rsEGFP2	0.35 ± 0.02	0.34 ± 0.01	0.3055	2.0	2.0	2.0	---
rsFastLime	0.62 ± 0.01	0.59 ± 0.01	0.6031, 0.7751	3.1	3.1	3.1	---

The error in all excited-state lifetime measurements did not exceed 0.1 ns.