Figure 3.

Prediction of healthy subjects and patients with gastric cancer using unknown samples and early-stage diagnosis. (A) For validation, 67 unknown samples (33 healthy control and 34 gastric cancer samples) were fitted into the OPLS-DA model. The class membership of the unknown samples was predicted using an a priori set cutoff value of 0.5 (dashed line). The Y-values of the black boxes and red triangles are from the analysis of known samples (67 healthy control and 68 gastric cancer samples), and gray triangles represent unknown samples. (B) For early-stage diagnosis, the gastric cancer group only comprised patients with stage IA cancer (n = 37), and the diagnostic model was established using the same healthy control group (n = 67). Validation was performed with 52 unknown samples (gray triangles). Black boxes: samples from healthy controls; red triangles: samples from patients with gastric cancer. In the case of misclassified samples, the predicted Y-values for unknown samples are shown as gray triangles.