Figure 1.

CRISPR/Cas12a/Cas9 target sites on the LacZα gene with various lengths of symmetrical HDR-NS oligonucleotides. The lacZα gene is shown with overlapping cleavage sites for CRISPR/Cas12a and CRISPR/Cas9. The staggered 5′, five base overhangs are generated by a Cas12a RNP and illustrated with a staggered red line while a Cas9 RNP generates a blunt ended cut shown by a dotted black arrow. Above the target sites are three oligonucleotides (denoted as the NS-strand in a 3′–5′ direction) with symmetrical homology arms of different lengths flanking an eight base NOTI site in the center.