Table 1.

Statistical comparison between CRISPR/Cas12a and CRISPR/Cas9 using homology arm lengths of 20, 50, and 75 bases. Fisher’s exact test was used to compare Cas12a with Cas9 using three different lengths of symmetrical homology arms. The total number of precise HDR events and Indels are presented along with the column totals and p-values < 0.05 for the three comparisons. p-values noted with * indicate statistically significant data within that comparison.

* Cas12a vs. Cas9-20HDR-NS
	HDR	Indel	Total
Cas12a 1364 20HDR-NS	42	22	64
Cas9 1364 20HDR-NS	20	41	61
Marginal column totals	62	63	125
p-value < 0.05				0.0003 *
* Cas12a vs. Cas9 50HDR-NS
	HDR	Indel	Total
Cas12a 1364 50HDR-NS	34	28	62
Cas9 1364 50HDR-NS	3	58	61
Marginal column totals	37	86	123
p-value < 0.05				0.0001 *
* Cas12a vs. Cas9-75HDR-NS
	HDR	Indel	Total
Cas12a 1364 75HDR-NS	26	30	56
Cas9 1364 75HDR-NS	2	61	63
Marginal column totals	28	91	119
p-value < 0.05				0.00001 *