Table 1.
Characteristics of the cell lines used in the study. If not otherwise specified, fetal bovine serum (FBS) was purchased from Bio&SELL (FBS Superior, #S 0615, Feucht, Germany).
| Cell Line | Origin | Catalog Nr | Medium | Supplements (Final Concentration) |
|---|---|---|---|---|
| PC3 | Adult, male Caucasian. Prostate, from metastatic site: bone. Adenocarcinoma, Grade IV |
CLS, cell line service #300312 |
ThermoFisher Scientific #11039047 |
2 mM L-glutamine, 5% FBS |
| LNCaP | Adult, male Caucasian. Prostate, from metastatic site: left supraclavicular lymph node. Carcinoma |
CLS, cell line service #300265 |
ThermoFisher Scientific #51200046 |
2 mM L-glutamine, 10% FBS, 0.1 mM nonessential amino acids, 1 mM sodium pyruvate |
| MDA-PCa-2b | Adult, male African American. Prostate, from metastatic site: bone | ATCC #CRL-2422 |
ATCC #30-2004 |
20% FBS, 25 ng/mL cholera toxin, 10 ng/mL mouse EGF, 0.005 mM phosphoethanolamine, 100 pg/mL hydrocortisone, 45 nM sodium selenite, 0.005 mg/mL human recombinant insulin |
| CWR-R1ca | Fibroblast-free cell line derived from the castration-resistant or recurrent CWR-R1 human prostate-cancer cell line | Merck-Millipore #SCC118 |
ThermoFisher Scientific #32404014 |
2 mM L-glutamine, 10% FBS |
| NCI-H660 | Adult, male Caucasian. Prostate, from metastatic site: lymph node |
ATCC #CRL-5813 |
ThermoFisher Scientific #32404014 |
4 mM L-glutamine, 5% FBS, 0.005 mg/mL insulin, 0.01 mg/mL transferrin, 30 nM sodium selenite, 10 nM hydrocortisone, 10 nM β-estradiol |
| DU145 | Adult, male Caucasian. Prostate, from metastatic site: brain |
DSMZ #ACC 261 |
ThermoFisher Scientific #32404014 |
2 mM L-glutamine, 10% FBS |
| HPEC | Adult, normal | Merck-Millipore #SCCE019 |
Merck-Millipore #SCMP001 (Kit) |
6 mM L-glutamine, 0.4% epiFactor P, 1 µM epinephrine, 0.5 ng/mL rh TGF-α, 100 ng/mL hydrocortisone hemisuccinate, 5 µg/mL human recombinant insulin, 5 µg/mL apo-transferrin |