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. 2020 Oct 6;9(10):2242. doi: 10.3390/cells9102242

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of P23H Flag-tagged rhodopsin at early stages of photoreceptor development in wild type (WT) and P23H transgenic (P23H) zebrafish. Expression of P23H mutant rhodopsin (Flag; red) and rhodopsin (Retp1; green) at 2 dpf (A,B), 3 dpf (C,D), 5 dpf (E,F), 7 dpf (G,H), and 9 dpf (I,J). Expression of Flag-tagged P23H mutant rhodopsin (red) compared to double cones (Zpr1, green) at 2 dpf (K,L), 3 dpf (M,N), 5 dpf (O,P), 7 dpf (Q,R), and 9 dpf (S,T). Yellow arrowheads denote cells expressing P23H mutant rhodopsin. Nuclei labeled with DAPI are blue. ONL: outer nuclear layer; INL: inner nuclear layer. Scale bar in L applies to all panels.