Figure 6.

Adhesion of B cells to stromal cells via clusterin expression. Clusterin mRNA expression by stromal cells and up-regulation in the FL context evaluated by Affymetrix probeset intensity (A) and confirmed by qRT-PCR (B), * p < 0.05; (C): CFSE-labeled FL-B cells were incubated for 2 h in wells pre-coated with or without (Ø) clusterin (left), or VCAM1-Fc chimera protein as a control (right). The number of adherent cells was quantified using a fluorescence reader. The number of adherent naive B cells in wells without any coated protein was assigned as 1.