Figure 5.

Effect of extra virgin olive oil (EVOO) extracts on acetic acid or oxytocin-induced writhing responses in mice. Mice were treated with EVOO extracts and ibuprofen (mg/kg, oral gavage), and we used (a) acetic acid injection, except for the control group, or (b) oxytocin injection. The amount of writhing was counted for 30 min after acetic acid administration. (c) 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) scavenging activity and (d) malondialdehyde (MDA) concentration. Data are presented as the mean ± standard error of mean (SEM) (n = 5). * p < 0.05 versus control group, # p < 0.05, ## p < 0.01, ### p < 0.001 versus vehicle group. C, Control; V, Vehicle (67 IU/kg oxytocin). ACN: Acetonitrile.