Table 3.

HPLC run condition for the samples.

	Curcuminoids	Lutein and Zeaxanthin	Vitamin D3
Column	Agilent Eclipse plus C18 (100 × 4.6) mm, 3.5 µm	Kromasil silica column (4 × 250) mm, 5 µm	Inertsil ODS C18 (250 × 4.6) mm, 5 μm
Wavelength	420 nm	446 nm	265 nm
Flow Rate	1.0 mL per minute	1 mL/minute	1 mL/min
Column oven Temp.	30 °C	25 °C	40 ± 2 °C
Sample oven temperature	25 °C	15 °C	20 °C
Injection volume	20 µL	20 µL	50 µL
Run time	15 min	20 min	20 min
Mobile phase	MP-A (Buffer): MP-B (THF) 600:400 ratio	hexane: ethyl acetate (500:500) v/v	600:400 (acetonitrile: methanol)
Diluent	Use the mobile phase	Mobile phase	Use the mobile phase