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. 2020 Oct 5;10(10):708. doi: 10.3390/brainsci10100708

Figure 1.

Figure 1

Expression and distribution of Pinin (Pnn) in primary cultured neurons and astrocytes with oxygen-glucose deprivation and reoxygenation. Western blotting was used to examine the protein expression of Pnn, histone deacetylase complex subunit (SAP18), and apoptosis-associated proteins (Bax, cleaved caspase-3, annexin V, caspase-independent apoptosis-inducing factor (AIF)) in neurons (A–F) and astrocytes (H–M) with oxygen-glucose deprivation and reoxygenation. Immunofluorescent stainings were used to examine the distribution of Pnn and SAP18 in neurons (G) and astrocytes (N) with oxygen-glucose deprivation. OGD, oxygen-glucose deprivation; OGD/R, oxygen-glucose deprivation and reoxygenation; AIF; caspase-independent apoptosis-inducing factor. * indicates p < 0.05 in comparison with control group; # indicated p < 0.05 in comparison with OGD group; n = 6 for each group. (G and N) scale bars in left lower corner of indicate 10 μm.