Figure 6.

Absolute activities of citrate synthase and OXPHOS complexes in isolated mitochondria from 300 nM ONC212- or vehicle-treated A375 cells. (a) Citrate synthase (CS); (b) Complex I (CI); (c) Complex II (CII); (d) Complex III (CIII); (e) Complex IV (CIV); (f) Complex V (CV). Values are given as mean ± SD. Unpaired T-test; * p ≤ 0.05; ** p ≤ 0.01. The activities of OXPHOS complexes were normalized to protein concentration in cell lysates. Measurements were repeated three times and each measurement includes two replicates (n = 3).