Figure 3.
Suppression of hypoxia-responsive gene expressions by AHX treatment. Relative fold changes in (A) Hif-1α, (B) Hif-2α, (C) Vegf, (D) Bnip3 and (E) Pdk1 mRNA expressions were shown after 6 h of AHX treatment under 200 µM CoCl2-induced pseudo-hypoxic condition in 661W cells by quantitative PCR analyses (n = 3–6 per group). The upregulated Vegf and Pdk1 mRNA expressions were suppressed by AHX treatment. ** p < 0.01, *** p < 0.001, # p < 0.05, ## p < 0.01 compared with no treatment and CoCl2 treatment, respectively. Bar graphs are presented as the mean with the ± standard error of the mean. The data were analyzed using one-way ANOVA followed by a Bonferroni post hoc test.