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Prediction of the 5′UTR secondary structure computed by RNAfold implemented in Geneious Prime 2020. The blue arrow indicates the stem-loop that is predicted to remain stable up to 64 °C with a free energy of the thermodynamic ensemble ∆G = −2.58 kcal/mol in 1 M NaCl. The color of the nucleotides is related to the base pair probabilities provided by RNAFold: red, very high; orange, high; blue, low.
