Table 2.
Primers used for mRNA quantitative real-time PCR.
| Gene | Forward Sequences (5′–3′) | Reverse Sequences (5′–3′) | Accession No. 1 | PCR Efficiency (%) | Product Length |
|---|---|---|---|---|---|
| Occludin | TGTCGGGGAGTGGGTAAA | TCCAGGCAAATAAAGAGGCT | XM_020599328.1 | 97 | 130 |
| ZO-1 | GGCATCATCCCCAACAAA | GCGAAGACCACGGAACCT | XM_020621576.1 | 96 | 111 |
| ZO-2 | AGCCGAGGTCGCACTTTA | GCTTTGCTTCTGTGGTTGAT | XM_020615114.1 | 98 | 246 |
| Claudin-12 | TCACCTTCAATCGCAACG | ATGTCTGGCTCAGGCTTATCT | XM_020607277.1 | 99 | 250 |
| Claudin-15 | CTCGCTGCTTGCTTTGACT | TTGAAGGCGTACCAGGACA | XM_020611334.1 | 96 | 225 |
| IL-10 | TTTGCCTGCCAAGTTATGAG | CATTTGGTGACATCGCTCTT | XM_020593225.1 | 100 | 158 |
| IL-1β | GAGATGTGGAGCCCAAACTT | CTGCCTCTGACCTTCTGGACTT | KM113037.1 | 97 | 127 |
| IL-12β | CAAGTCAGTTGCCAAAATCC | CCAAGCAGCTCAGGGTCT | XM_020594580.1 | 99 | 103 |
| TGF-β1 | AACCCACTACCTCACTACCCG | GCCGAAGTTGGAAACCCT | XM_020605575.1 | 96 | 128 |
| TGF-β2 | ATTACGCCAAGGAGGTGC | GGGTTTTGAAGACGGAAGAT | XM_020622328.1 | 98 | 178 |
| TGF-β3 | AGTTTGTCGCTATCCACTTGC | GATGAGTTCCTTGGTGCTGTTA | XM_020590885.1 | 95 | 180 |
| TLR-3 | TATTTAGAGCCATACAGGG | CACAATCAAGAACGCACA | XM_020614353.1 | 100 | 244 |
| TLR-7 | ATCCTCACGACTTCCCTC | TTTCTTTCATCACCCACT | XM_020596482.1 | 97 | 205 |
| TLR-8 | AAGTGAAGCAGGATGAAG | AAGTCCCAGATTGAGTGA | XM_020596483.1 | 96 | 139 |
| Nrf2 | CTTCAGACAGCGGTGACAGG | GCCTCATTCAGTTGGTGCTT | XM_020596409.1 | 96 | 260 |
| Keap1 | AGCCTGGGTGCGATACGA | CAAGAAATGACTTTGGTGGG | XM_020597068.1 | 98 | 198 |
| SOD1 | AGCTGGCTAAGTTCTCATTCAC | GCAGTAACATTGCCCAAGTCT | XM_020598413.1 | 99 | 227 |
| CAT | GTCCAAGTCTAAGGCATCTCC | CTCCTCTTCGTTCAGCACC | XM_020624985.1 | 96 | 106 |
| GPx1 | GTTCACCGCCAAACTCTT | TTCCCATTCACATCTACCTT | XM_020607739.1 | 98 | 303 |
| GPx4 | ATTTATGACTTCTCAGCGACAG | CCTTCAGCCACTTCCACA | XM_020612291.1 | 100 | 325 |
| GSTK | TTGATGTTCCCCTGCGTTAT | CACCTGCTCTACCTGCTTGTC | XM_020610780.1 | 97 | 131 |
| GSTO | GGGAGAAATAAAGGTGAGGATG | CAGATGAGTTGACAAGGCAGTT | XM_020600427.1 | 99 | 199 |
| RPL-17 | GTTGTAGCGACGGAAAGGGAC | GACTAAATCATGCAAGTCGAGGG | XM_020587712.1 | 98 | 160 |
1 The accession numbers come from National Center for Biotechnology Information (NCBI).