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. 2020 Oct 3;12(10):3037. doi: 10.3390/nu12103037

Figure 2.

Figure 2

Figure 2

Inula britannica flower aqueous extract (IAE) exerts anti-adipogenic effects in MDI-induced differentiation of 3T3-L1 preadipocytes involving regulation of Akt/GSK-3β signaling pathways. The cells were differentiated for eight days with the indicated concentrations of IAE. The phosphorylation of Akt (Ser 473) and GSK-3β (Ser21/9) was examined to evaluate the activation of the Akt/GSK-3β signaling pathways. The protein expression levels of adipogenesis-associated biomarkers were determined by using western blotting. (A) Protein expression levels of lipogenesis-associated biomarkers. (B) Relative protein expression levels of lipogenesis-associated biomarkers. (C) Protein expression levels of adipogenesis-specific biomarkers. (D) Relative protein expression levels of adipogenesis-specific biomarkers. (E) Effect of IAE on the activation of Akt/GSK-3β signaling pathways. (F) Relative protein expression levels of phosphorylated Akt and GSK-3β. The data are presented as mean ± standard deviation. Values labeled with different letters (a–e) are significantly different (p < 0.05). Differences among the multiple groups were determined based on one-tailed one-way analysis of variance, followed by Tukey’s post hoc test.