Figure 3.

Inula britannica flower aqueous extract (IAE) affects the early phases of adipogenesis. During the eight days of 3T3-L1 differentiation, IAE (200 μg/mL) was treated at different time points as indicated. (A) Schematic depiction of different treatment intervals of IAE during 3T3-L1 differentiation. (B) Intracellular lipids were stained with Oil Red O on day 8. (C) Relative absorbance of Oil Red O dye eluted from intracellular lipids at 500 nm. (D) Expression levels of adipogenesis-associated proteins on day 0, 2, 4, and 6 of differentiation. The data are presented as mean ± standard deviation. Values labeled with different letters (a–d) are significantly different (p < 0.05). Differences among the multiple groups were determined based on one-tailed one-way analysis of variance, followed by Tukey’s post hoc test.