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. 2020 Oct 4;8(10):1524. doi: 10.3390/microorganisms8101524

Figure 6.

Figure 6

Treatment with curcumin reduces HAdV types 4 and 7 protein levels. A549 cells were infected with HAdV-4 or -7 at an MOI of 10 for 1 h, and overlaid with medium containing curcumin (0, 25, 50, and 100 μM). Twenty-four hpi, crude cell protein extracts were prepared and analyzed by immunoblot for late protein levels. (A) Samples infected with HAdV-4 were analyzed for expression of several late HAdV proteins by immunoblot using an α-HAdV-5 antibody. (B) Quantification of penton protein from HAdV-4 (~63 kDa major band) normalized to tubulin. Values are presented relative to vehicle-treated cells. (C) Samples infected with HAdV-7 were analyzed for expression of several late HAdV proteins by immunoblot using an α-HAdV-5 antibody. (D) Quantification of penton protein from HAdV-7 (~63 kDa major band) normalized to tubulin. Values are presented relative to vehicle-treated cells. The mean of three experiments are shown, and the error bars represent standard deviation (SD) of the mean. * p ≤ 0.05, **** p ≤ 0.0001.