Table 1.
Physicochemical properties of superparamagnetic iron oxide nanoparticles (SPION) and mitoxantrone-loaded ones (SPIONMTO) adapted from [28,29], Elsevier, 2018, 2016.
| Physicochemical Properties |
Method | SPION | SPIONMTO |
|---|---|---|---|
| Hydrodynamic size (nm) and polydispersity index |
Dynamic light scattering (DLS) in RPMI 1640 (Malvern Zetasizer NanoZS) | 52.7 ± 0.8 nm; 0.202 ± 0.026 |
55.7 ± 1.0 nm; 0.239 ± 0.019 |
| Core size (nm) | Freeze fracture TEM | 10 nm iron core size with 2 nm organic layer | n.d. |
| Zeta potential (mV) | Malvern Zetasizer NanoZS; in RPMI 1640 medium | −11.9 ± 0.8 | −12.4 ± 0.5 |
| Drug loading efficacy (%) (200 µg mitoxantrone (MTO) per 1 mL SPIONs) |
High-performance liquid chromotograpy (HPLC)-UV of supernatant to detect unbound drug | 98.9 ± 0.2% | |
| MTO release (%) after 72 h at 37 °C in RPMI 1640 medium | (1) Dialysis bag method (2) Magnetic assay |
11.6 ± 0.1% 23.7 ± 0.4% |
|