Table 1.
Primers sequence used for real-time PCR for gene expression analysis.
| TLR Pathway Mediators | Accession Number | Primer Sequence (5′ to 3′) | |
|---|---|---|---|
| Forward | Reverse | ||
| Keap1 | NM_057152.2 | CTTCGGGGAGGA GGAGTTCT | CGTTCAGATCATCGCGGCTG |
| Nrf2 | NM_031789.2 | CATTTGTAGATGACCATGAG TCGC | ATCAGGGGTGGTGAAGACTG |
| HO-1 | NM_012580.2 | GTGCACATCGTGCAGAGAA | GTGCACATCCGTGCAGAGAA |
| PI3K | NM_001371300.1 | GCCCAGGCTTACTACAGAC | AAGTAGGGAGGCATCTCG |
| Akt | NM_033230.2 | CGCCTGCCCTTCTACAACC | TCATACACATCTTGCCACACGA |
| mTOR | NM_019906.1 | CTGTAATTACATCCTCGACTG | CGTGTCGTGGTTAGTCG |
| Bax | NM_017059.2 | GTGGTTGCCCTCTTCTACTTTG | CAAAAGATGGTCACTGTCTGC |
| Bcl-2 | NM_016993.1 | CCGGGAGATCGTGATGAAGT | ATCCCAGCC TCCGTTATCCT |
| β-actin | NM_031144.3 | CACGATGGAGGGGCCGGACTCATC | TAAAGACCTCTATGCCAACACAGT |