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. 2020 Sep 30;12(10):2822. doi: 10.3390/cancers12102822

Figure 3.

Figure 3

Detection of original mutations (L858R, 19del) and acquired T790M by ddPCR in BW-derived EV-DNA with plasma-derived cfDNA and tissue samples. (A) In 11 patients with re-biopsy samples, T790M was detected in 61.5%, 10%, and 30.8% of EV-DNA in BW, cfDNA of plasma, and tissues, respectively. Patients 1 (P1) and 5 (P5) were included in the longitudinal study and additional samples were collected after four and eight months for P1 (P1-1) and P5 (P5-1), respectively. (B–E) In samples of P2 (B,C) and P5-1 (D,E), T790M was detected in BW-derived EV-DNA samples but not in tissue samples. After three or four months, the acquired T790M mutation was detected from plasma cfDNA (P2-1) and both from plasma cfDNA and tissue re-biopsy samples (P5-2), respectively. CT scan images showed that the tumor size slightly increased (C) from 9.0 (P2) to 9.6 cm (P2-1) and (E) from 7.1 (P5-1) to 7.3 cm (P5-2) during the three and four months for P2 and P5-1, respectively.