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. 2020 Sep 30;12(10):2822. doi: 10.3390/cancers12102822

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Monitoring of lung cancer patients via CT scan image and molecular analysis of EGFR mutations by ddPCR of BW-derived EV-DNA. (A) The size of the tumor decreased from 8.3 (P5) to 7.1 cm (P5-1) after about four months. However, a new metastasized lesion was found in the brain. (B) 19del EGFR mutation was increased and T790M EGFR mutation was emerged. (C) The tumor size increased from 3.7 to 5.6 cm after eight months of TKI therapy. (D) The L858R EGFR mutation was also increased, and T790M EGFR mutation emerged from ddPCR results of BW-derived EV-based liquid biopsy.