Figure 8.

Expression levels of virulence-related genes in Pectobacterium strains (A) PC16 and (B) WPP14 in response to phenolic extracts from calla lily or cabbage leaves. The transcript levels of the examined genes were analyzed in DNA-free RNA from the bacterial strains PC16 and WPP14 after 8 h in LB medium with or without host extracts (control), at 28 °C under continuous shaking (150 rpm). The expression levels of expI, expR, pel, peh, hrpL and araF were quantified relative to control using qRT-PCR. Experiments were repeated three times with three replicates showing similar trends. Bars represents mean ± SE of relative expression of each gene. Tukey–Kramer HSD test was used to test the differences, bars not labeled with the same letter (a–c) are significantly different from each other (p < 0.05).