Figure 1.

NS1 is secreted at undetectable levels in mosquito cell lines. Vero and C6/36 cells were infected with dengue virus (DENV) 2 at multiplicity of infection (MOI) = 0.5. After two days of incubation, culture supernatants and cells were harvested. The cells were lysed with 1% Triton X-100-phosphate-buffered saline (PBS) buffer. (A) Cell culture supernatant (20 µL) and (B) cell lysate (10 µg) were subjected to immunoblotting with non-structural protein 1 (NS1) antibody.