Figure 3.

DENV2 progression in infected mosquito bodies. Mosquitoes were infected orally (A) or via thorax injection (B) with DENV2 and then incubated. At days three, five and seven post-infection (PI), mosquitoes were harvested and dissected to obtain midgut (MG), fat, muscle and salivary glands (SG). All of these tissues were stained with anti-NS1 Ab (red), anti-E Ab (green) and DAPI (blue). The processed tissues were analyzed using confocal microscopy.