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NS1 distribution could be different in intrathoracically and orally infected mosquitoes. Mosquitoes were infected intrathoracically or orally with DENV2. After homogenization, NS1 could be detected in both PBS and Triton X-100-PBS lysates of intrathoracically infected mosquitoes; however, in the orally infected mosquitoes, NS1 could be detected only following Triton X-100-PBS treatment.
