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. 2020 Oct 2;9(10):2232. doi: 10.3390/cells9102232

Figure 1.

Figure 1

ProNGF and p75NTR are expressed in satellite cell-derived myoblasts (SCDMs) under both proliferating and differentiating conditions. (A) Western blot and densitometric analysis of p75NTR full length (p75NTR FL) and p75NTR intracellular domain (p75NTR ICD) in the total lysates of SCDMs cultured in the proliferation medium (PM) and differentiation medium (DM) for 24 h, derived from three independent experiments. (B) Western blot and densitometric analysis of proNGF in the total lysates of SCDMs cultured as in A. N = 3 independent experiments. Tubulin was employed as a loading control. (C) p75NTR immunofluorescence staining (red) in SCDMs kept in DM for 24 h. DAPI was used to counterstain nuclei. N = 3 independent experiments. (D) Western blot of p75NTR performed on nuclear extracts from SCDMs, 24 h after DM addition. H3 and GAPDH were used as protein loading controls and as nuclear and cytoplasmic markers, respectively. N = 3 independent experiments. Values are represented as the mean ± SD. Statistical analysis was performed by using the Student’s t test. * p < 0.05; † p < 0.01. C = cytosolic fraction; N = nuclear fraction.