Figure 4.

Effects of proNGF administration on the slow/oxidative markers during SCDM differentiation. (A) Western blot and densitometric analysis of (B) slow myosin heavy chain (slow MyHC), (C) fast MyHC), (D) cytrochrome C, (E) COX IV and (F) a nuclear and transcriptionally active fragment of SREBP-1 (nSREBP-1). SCDMs were allowed to differentiate in the differentiation medium, and treated with vehicle (PBS) or proNGF (100 ng/mL). Then, 6, 24 and 48 h after the induction of the differentiation, the cells were harvested and the total lysates were performed to evaluate the protein expression by Western blot. Tubulin was chosen as the housekeeping protein to normalize the protein loading. Data are expressed as the mean ± SD, and derived from three independent experiments. Statistical analysis was performed by using two-way ANOVA. * p < 0.05; ^† p < 0.01; ^‡ p < 0.001.