Skip to main content
. 2020 Oct 2;9(10):2232. doi: 10.3390/cells9102232

Figure 5.

Figure 5

The evaluation of p75NTR localization and signaling cascades involved in the regulation of muscle fiber metabolism in differentiating SCDMs treated with proNGF. (A) SCDMs were induced to differentiate into the differentiation medium, and were treated with vehicle (PBS) or proNGF (100 ng/mL). Six hours after treatment, SCDMs were fixed and processed to carry out immunofluorescence analysis for p75NTR (red). DAPI was employed to visualize the nuclei. Nuclear intensity derived from p75NTR immunopositivity was analyzed by ImageJ and reported as arbitrary units (a.u.). N = 3. (B) Western blot and the densitometric analysis of (C) CREB phosphorylation at Ser133 (p-CREB), (D) AMPK phosphorylation at Thr172 (p-AMPK) and (E) PGC-1α. Differentiating SCDMs were treated with vehicle (PBS) or proNGF (100 ng/mL). Cells were collected at 6, 24, and 48 h and protein expression by means of Western blot was assessed with the total lysates. Tubulin was used as the housekeeping protein to normalize the protein loading. Results are expressed as the mean ± SD, and were obtained from three independent experiments. Statistical analysis was performed by using two-way ANOVA. * p < 0.05; † p < 0.01; ‡ p < 0.001.