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. 2020 Oct 2;9(10):2232. doi: 10.3390/cells9102232

Figure 7.

Figure 7

Effects of the proNGF/p75NTR pathway on mRNA levels of slow/oxidative and fast/glycolytic markers in the differentiated myotubes. qRT-PCR analysis of (A) the slow/oxidative markers and of (B) the fast/glycolytic markers in differentiated myotubes treated with proNGF or proNGF + LM11A-31. SCDMs were differentiated into myotubes for 72 h, and then treated with vehicle (PBS), proNGF (100 ng/mL) and proNGF (100 ng/mL) + LM11A-31 (100 nM) for 48 h. (C) C2C12 myotubes were treated as described above, and the transcript levels were evaluated by qRT-PCR. mRNA levels of slow myosin heavy chain 7 (Myh7), cytochrome C (Cycs), succinate dehydrogenase (Sdha), mitochondrial transcription factor A (Tfam), cytochrome c oxidase I (MT-COI), fast myosin heavy chain 2B (Myh4), troponin C2 (Tnnc2) and T-box transcription factor 15 (Tbx15) are reported as an increase with respect to the control (CTRL) cells treated with vehicle (PBS). Data are normalized against hprt, chosen as the reference gene. Statistical analysis was performed by using one-way ANOVA. * p < 0.05; † p < 0.01. Arbitrary units (a.u.).