Figure 8.

(a) 3D correlation spectra of deuterated NS4B from HCV with sequential connectivities. Selected strip plots representing the assignment of amino acid stretches of residues 119 to 125 and 237 to 241 using a set of 6 spectra recorded at 110 kHz MAS on two NS4B samples with different labeling schemes [60]. The orange spectrum was acquired at 60 kHz MAS. (b) A putative topology model of the NS4B protein adapted from [150], in which NS4B is proposed to contain four presumably amphipathic α-helices and four predicted transmembrane segments in the middle. The black boxes indicate the location of the two assigned regions shown in panel a. Figure reproduced with permission from reference [60].