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. 2020 Sep 26;9(10):791. doi: 10.3390/pathogens9100791

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Reference DNA amplification of specific polymerase chain reaction (PCR) products of Echinococcus granulosus sensu lato. PCR products from reference DNAs obtained from Echinococcus granulosus sensu lato species amplified by specific PCR (Cest4, Cest5 (Trachsel et al., 2007)). 1: E. granulosus sensu stricto (G1), 2: E. granulosus s.s. (G3 ), 3: E. granulosus s.s. (G3), 4–5: E. granulosus s.s. (Gx), 6–7: E. equinus (G4), 8–10: E. ortleppi (G5), 11–12: E. canadensis (G6), 13: E. canadensis (G7), 14: E. canadensis (G8), 15–16: E. canadensis (G10), 17: E. vogeli, 18: E. felidis, 19: E. cf. granulosus (G-Omo). M: 100 base pair (bp) marker. NC: non-template control, EC: negative extraction control, PK: positive control. Blue vertical lines: gel spliced to clean up image and for labelling purposes.