Table 3.
Analytical sensitivity and efficiency of developed TaqMan® qPCRs.
| PCR Name | Specificity | Efficiency with Cloned PCR Products | Efficiency Clinical DNA | Detection Limit (Copy Number/µL at Cq) with Cloned PCR Products | Detection Limit in Cq with Clinical DNA |
|---|---|---|---|---|---|
| G1_3_qPCR (single-plex) | E. granulosus s.s. (G1, G3) | 100.7% (R2 = 0.99, slope = −3.34) | 96.3% (R2 = 0.99, slope = −3.41) | 0.8/µL at Cq 36 (± 0.9) | 38.24 (± 0.1) |
| G4_qPCR (single-plex) | E. equinus (G4) | 106.7% (R2 = 0.99, slope = −3.32) | 93.7% (R2 = 0.99, slope = −3.48) | 0.6/µL at Cq 39 (± 0.6) | 38 (± 1) |
| G5_G10_qPCR (duplex) | E. ortleppi (G5) | 100.7% (R2 = 0.99, slope = −3.34) | 97.3% (R2 = 0.99, slope = −3.39) | 1.4/µL at Cq 36 (± 0.7) | 37 (± 0.4) |
| G5_G10_qPCR (duplex) | E. canadensis (G6–8, G10) | 101.6% (R2 = 0.99, slope = −3.28) | 99.9% (R2 = 0.99, slope = −3.33) | 1.4/µL at Cq 36 (± 0.8) | 38.6 (± 0.17) |