Table 4.

Detection of E. granulosus s.l. species in faecal samples by duplex and triplex TaqMan^® qPCRs.

PCR Name	Specificity	1:100 §	1:100 *	1:1000 *	1:10,000 *	1:100,000 *
G1_3_qPCR+IC_qPCR (duplex)	G1, G3	24.5	30.1 (± 0.6)	35.2 (± 1)	neg.	neg.
G4_qPCR+IC_qPCR (duplex)	G4	20.2	27.4 (± 0.5)	30.7 (± 1)	35.8 (± 0.06)	neg.
G5_10_qPCR+IC_qPCR (triplex)	G5	21,37	30.3 (± 0.17)	32.4 (± 0.22)	neg.	neg.
G5_10_qPCR+IC_qPCR (triplex)	G6–8, G10	24.2	31.2 (± 0.02)	32.6 (± 0.22)	neg.	neg.

^§ Cq values from 1:100 pre-diluted DNA, extracted from cyst material. * Cq values from re-extracted DNA prepared by logarithmic dilution of the corresponding DNA, used to spike faecal samples (± standard deviation).