Figure 3.

NMR studies of HIV-1 MA structure and membrane targeting. (A) ¹H-¹⁵N HSQC spectra of ¹⁵N-labeled MA titrated into bicelles containing increasing mol% native PI(4,5)P₂, reveal the binding mode of MA to plasma membrane mimetics. (B) Solution structure of MA determined by NMR. Residues showing large chemical shift perturbations in (A) are highlighted. (C) ¹H NMR spectra of MA and myr(-) MA upon addition of liposomes with varying phospholipid compositions. Signal loss indicates binding. MA can bind non-Raft-like membranes due to myristoyl group interactions. Binding to Raft-like membranes is significantly enhanced. Adapted from [122] with permission.