Figure 14.

Improved NMR spectral quality afforded by ²H-edited NMR in the studies of MoMuLV minimal packaging signal, with loop residues engineered to prevent dimerization (mΨ^CES). (A) Representative strips from 3D ¹H-¹³C correlated with the NOESY spectra obtained for ¹³C-labeled mΨ^CES. (B) Portion of a 2D NOESY spectrum obtained for an mΨ^CES sample containing protonated guanosines and with all other predeuterated nucleotides (breakthrough signals from incomplete deuteration of adenosines are also visible). Linewidths are significantly narrower in the ²H-edited spectra. (C) 3D NMR structure of mΨ^CES. Adapted from [322], with permission.