Figure 5.

Effects of circulating polyR-Fe₃O₄ on enthodelial cell grown under flow conditions. HUVECs were grown in bifurcating slides until confluence and perfused with polyR-Fe₃O₄-containing medium for 18 h. The representative laminar and non-uniform regions are shown after fluorescent staining. Nuclei are visualized using a Hoechst 33342 (blue) staining, whereas F-actin is visualized with Alexa 488-conjugated phalloidin (green). Representative images of n = 3 experiments are shown. (Scale bars: 50 μm).