Table 2.
Results (Cq values) of EDTA-blood, serum, nasal and oral swabs taken from goats inoculated with GTPV-“V/103“ analyzed via pan Capripox real-time qPCR.
| GTPV-“V/103“ | Capri-p32-Mix 1-Taq-FAM | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| dpi | ||||||||||||
| 0 | 3 | 5 | 7 | 10 | 13 | 15 | 18 | 21 | 23/24 | 28 | ||
| Animal | matrix | |||||||||||
| Z/253 i.v. + s.c. | EDTA-blood | no Cq | no Cq | 36.4 | 34.3 | 32.1 | - | - | - | - | - | - |
| serum | no Cq | no Cq | no Cq | no Cq | 37.5 | - | - | - | - | - | - | |
| nasal swab | no Cq | no Cq | 39.5 | no Cq | 27.9 | - | - | - | - | - | - | |
| oral swab | no Cq | no Cq | no Cq | no Cq | 34.8 | - | - | - | - | - | - | |
| Z/254 i.v. + s.c. | EDTA-blood | no Cq | no Cq | 36.6 | 26.8 | 27.2 | - | - | - | - | - | - |
| serum | no Cq | no Cq | no Cq | 37.7 | 34.9 | - | - | - | - | - | - | |
| nasal swab | no Cq | no Cq | no Cq | 37.0 | 26.6 | - | - | - | - | - | - | |
| oral swab | no Cq | no Cq | no Cq | no Cq | 32.2 | - | - | - | - | - | - | |
| Z/255 i.v. + s.c. | EDTA-blood | no Cq | no Cq | 34.4 | 30.1 | 27.5 | - | - | - | - | - | - |
| serum | no Cq | no Cq | no Cq | no Cq | 36.3 | - | - | - | - | - | - | |
| nasal swab | no Cq | no Cq | 36.1 | no Cq | 28.4 | - | - | - | - | - | - | |
| oral swab | no Cq | no Cq | no Cq | no Cq | 34.4 | - | - | - | - | - | - | |
| Z/256 i.n. | EDTA-blood | no Cq | no Cq | no Cq | no Cq | 30.3 | 29.2 | 31.2 | - | - | - | - |
| serum | no Cq | no Cq | no Cq | no Cq | no Cq | 35.6 | 37.9 | - | - | - | - | |
| nasal swab | no Cq | no Cq | 27.02 | 23.6 | 18.9 | 19.0 | 19.6 | - | - | - | - | |
| oral swab | no Cq | no Cq | no Cq | 28.6 | 27.4 | 26.0 | 25.7 | - | - | - | - | |
| Z/257 i.n. | EDTA-blood | no Cq | no Cq | no Cq | 34.4 | 33.6 | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq |
| serum | no Cq | no Cq | no Cq | n.a. | no Cq | no Cq | n.a. | no Cq | no Cq | no Cq | no Cq | |
| nasal swab | no Cq | 37.7 | 26.3 | 21.4 | 19.9 | 19.1 | 20.4 | 21.5 | 22.4 | 26.6 | 26.1 | |
| oral swab | no Cq | no Cq | 36.9 | 32.0 | 27.8 | 27.7 | 26.4 | 30.2 | 29.6 | 32.1 | 36.1 | |
| Z/258 i.n. | EDTA-blood | no Cq | no Cq | no Cq | no Cq | 33.0 | 35.0 | no Cq | no Cq | no Cq | no Cq | no Cq |
| serum | no Cq | no Cq | no Cq | no Cq | 37.4 | 37.2 | no Cq | no Cq | no Cq | no Cq | no Cq | |
| nasal swab | no Cq | 38.1 | 29.4 | 24.0 | 24.0 | 19.8 | 21.5 | 25.4 | 25.0 | 24.4 | 33.4 | |
| oral swab | no Cq | no Cq | 36.8 | 33.6 | 31.2 | 31.0 | 28.9 | 29.8 | 32.7 | 31.2 | 35.7 | |
| Z/259 i-c | EDTA-blood | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | 29.8 | 27.8 | - |
| serum | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | 37.3 | 31.4 | - | |
| nasal swab | no Cq | no Cq | no Cq | no Cq | no Cq | 34.1 | 33.8 | 35.1 | 21.1 | 20.5 | - | |
| oral swab | no Cq | no Cq | no Cq | no Cq | no Cq | 34.4 | 36.0 | 33.4 | 33.6 | 30.3 | - | |
| Z/260 i-c | EDTA-blood | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | 35.0 | 29.6 |
| serum | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | no Cq | 35.9 | |
| nasal swab | no Cq | no Cq | no Cq | no Cq | 31.4 | 36.4 | 33.7 | 33.5 | 31.8 | 34.1 | 20.4 | |
| oral swab | no Cq | no Cq | 35.8 | no Cq | 37.3 | 33.3 | 37.8 | 31.7 | 33.9 | 33.9 | 27.9 | |
-means animal was removed from the trial before sampling; n.a.—displays sample not analyzable.