Figure 4.

Effects of oral administration of KRG and KRG-P on α-defensin-1 expression in mouse intestinal tissues. BALB/c mice were orally administered the indicated doses of KRG or KRG-P daily for 10 days. (A) e α-defensin-1 protein levels were determined by immunoblotting with the specific antibody. Actin was used as a loading control. The bar chart displays the intensity of α-defensin-1 after normalizing with that of β-actin by using ImageJ software. (B) α-defensin-1 mRNA levels were determined by using qRT-PCR. An actin gene was used as a housekeeping gene for normalization. Data are presented as the means ± SD of three independent experiments. * p < 0.05 vs. the normal group.