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. 2020 Sep 27;9(10):797. doi: 10.3390/pathogens9100797

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) Flow diagram of inverse PCR. (b) Gel electrophoresis of inverse PCR products from Tn5 transposon mutants using specific primers KAN-2 FP-1 F and KAN-2 RP-1 R. M: 2000 bp DNA marker; 1–11: Tn5-30, -45, -63, -145, -146, -158, -216, -225, -273, -331, -355. (c) Verification of Tn5 insertion site by using site-specific primers. M: 2000 bp DNA marker; 1–22: wild type (1, 3, 5, 7, 9, 11, 13, 15, 17, 19 and 21) and Tn5 transposon mutants (2, 4, 6, 8, 10, 12, 14, 16, 18, 20 and 22) of Tn5-30, -45, -63, -145, -146, -158, -216, -225, -273, -331, -355, respectively.