Table 1.
Identification of Tn5 transposon insertion sites by inverse polymerase chain reaction (PCR) amplification and the alignment of the flanking sequences with strain R456 genome and Tn5 transposon sequence.
| Mutants | Locus_Tag | Enzyme Site | Sequence Length (bp) | Function Prediction |
|---|---|---|---|---|
| Tn5-30 | BsemR456_1725 | SalI | 861 | glycosyl transferase |
| Tn5-45 | BsemR456_1417 | PaeI | 203 | multispecies: histone H1 |
| Tn5-63 | BsemR456_2057 | SalI | 479 | nonribosomal peptide synthetase |
| Tn5-145 | BsemR456_1423 | SalI | 55 | 50S ribosomal protein L11 methyltransferase |
| Tn5-146 | BsemR456_6327 | SalI | 150 | Plasmid, membrane integrity-associated transporter subunit PqiC |
| Tn5-158 | BsemR456_6210 | SalI | 452 | Plasmid, hypothetical protein |
| Tn5-216 | BsemR456_1157 | SalI | 765 | tRNA uridine-5-carboxymethylaminomethyl (34) synthesis enzyme MnmG. |
| Tn5-225 | BsemR456_2679 | PaeI | 33 | YeiH family putative sulfate export transporter |
| Tn5-273 | BsemR456_1556 | SalI | 281 | catalase/peroxidase HPI |
| Tn5-331 | BsemR456_315 | SalI | 1173 | sulfate adenylyltransferase subunit CysD |
| Tn5-355 | BsemR456_6210 | SalI | 452 | Plasmid, hypothetical protein |
The function of these genes was predicted based on the website of NCBI BLAST.