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Encapsulation of cancer cells by fibroblast spheroids in 3D Matrigel. CaKi-1, HeLa, A375 and A549 were co-cultured with BHK-21 in 50% Matrigel for a period of up to 30 days. As shown by the fluorescent microscope images, almost the entire cancer cell population was encapsulated within the spheroids by the end of day 24.
