Table 2.
Ovarian cancer Tn-glycoproteins carrying the MGL binding motif.
| Function | Gene | Protein | Glycopeptide Sequences | Glycan Moieties | Glycosylated Sites * |
|---|---|---|---|---|---|
| Mucins | MUC1 | Mucin-1 | SGHASSTPGGEKETSATQR | 4 | S6; T7; T14; S15 |
| SSTPGGEKETSATQR | 4 | S2; T3; T10; S11 | |||
| MUC16 | Mucin-16 | FLHSEMTTLMSR | 2 | T7; T8 | |
| IHPSSNTPVVNVGTVIYK | 4 | S4; S5; T7; T14 | |||
| ISTPDHDKSTVPP | 4 | S2; T3; S9; T10 | |||
| MUC24 | Mucin-24 | VTTPAPETCEGR | 2 | T2; T3 | |
| Protein folding/cell metabolism | ERP44 | Endoplamic Reticulum Resident Protein 44 | EFHHGPDPTDTAPGEQAQDVASSPPESSFQK | 2 | S22; S23 |
| LAMP1 | Lysosome-associated membrane glycoprotein 1 | CEQDRPSPTTAPPAPPSPSPSPVPK | 5 | T9; T10; S17; S19; S21 | |
| RPSPTTAPPAPPSPSPSPVPK | 5 | T5; T6; S13; S15; S17 | |||
| LAMP2 | Lysosome-associated membrane glycoprotein 2 | TSTVAPTIHTTVPSPTTTPTPK | 7 | T3; T7; T10; T11; T16; T17; T18 | |
| QSOX1 | Sulphydryl oxidase 1 | EAAQTTVAPTTANK | 4 | T5; T6; T10; T11 | |
| Protein folding/cell metabolism | SEL1L | Protein Sel-1 homolog 1 | TTLTSDESVKDHTTAGR | 2 | T13; T14 |
| LRR8CD | Leucin-rich repeated-containing protein 8D | AHTPPGNAEVTTNIPK | 3 | T3; T11; T12 | |
| Matrix/cell interaction | AGRN | Agrin | ATTASRLPSSAVTPR | 4 | T2; T3; S10; T13 |
| APHPSHTSQPVAKTTAAPTTR | 5 | T7; S8; T14; T15; T19 | |||
| APP | Amyloid beta A4 protein | GLTTRPGSGLTNIK | 3 | T3; T4; T11 | |
| DAG1 | Dystroglycan | IRTTTSGVPR | 4 | T3; T4; T5; S6 | |
| FN1 | Fibronectin | HTSVQTTSSGSGPFDVR | 2 | T6;T7 | |
| NID-2 | Nidogen-2 | SYPASGHTTPLSR | 2 | T8; T9 | |
| PODXL | Podocalyxin | SSHSVTTDLTSTK | 7 | S2; S4; T6; T7; T10; S11; T12 | |
| SDC3 | Syndecan-3 | LVSTATSRPR | 3 | S3; T4; S7 | |
| TPTPETFLTTIR | 3 | T6; T9; T10 | |||
| VCAN | Versican core protein | HLVTTVPKDPEAAEAR | 2 | T4; T5 | |
| YLSTTPFPSQHR | 2 | T4; T5 | |||
| QESSTTFVSDGSLEKHPEVPSAK | 3 | T5; T6; S21 | |||
| STILPTAEVEGTKAPVEKEEVK | 4 | S1; T2; T6; T12 | |||
| STILPTAEVEGTKAPVEK | 4 | S1; T2; T6; T12 | |||
| LHTTSAFKPSSAITK | 4 | T3; T4; S11; T14 | |||
| RKEEEGTTGTASTFEVYSSTQR | 4 | T7; S12; S18; S19 | |||
| TTDYSVLTTKK | 4 | T1; T2; T8; T9 |
Tn-glycopeptide sequences were found in both tumor and ovarian cell cancer cell lines. *: T and S: threonine and serine amino acid residues, respectively.