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Control dog. Immunostains for the C-terminus (A), rod domain (B), and N-terminus (C) of the dystrophin in the diaphragm of a normal dog showing diffuse and strong sarcolemmal staining (positive control, transverse sections, objective 20×). In the affected dog, there was a total loss of positivity using both the anti-C-terminal (D) and N-terminal antibodies (F), and a very mild and multifocal signal with the anti-Rod antibody (E) (transverse sections, objective 20×).
