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. 2020 Oct 5;18(10):506. doi: 10.3390/md18100506

Figure 6.

Figure 6

Chiral high-performance liquid chromatography (HPLC) analysis of free astaxanthin from Tigriopus californicus fed with meso-zeaxanthin. (A) Astaxanthin standard (racemic mixture) with the typical peaks of 3S,3′S-astaxanthin (SA), 3R,3′S-(meso)-astaxanthin (MA), and 3R,3′R-astaxanthin (RA). (B) HPLC chromatogram of the carotenoid extract of T. californicus fed with yeast. (C). HPLC chromatogram of carotenoid extract of T. californicus fed with marigold powder rich in meso-zeaxanthin. Peak V is violaxanthin. (D) Spectra of astaxanthin peaks detected in the HPLC analysis.