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. 2020 Oct 5;18(10):506. doi: 10.3390/md18100506

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Chiral high-performance liquid chromatography (HPLC) analysis of free astaxanthin from Tigriopus californicus fed with meso-zeaxanthin. (A) Astaxanthin standard (racemic mixture) with the typical peaks of 3S,3′S-astaxanthin (SA), 3R,3′S-(meso)-astaxanthin (MA), and 3R,3′R-astaxanthin (RA). (B) HPLC chromatogram of the carotenoid extract of T. californicus fed with yeast. (C). HPLC chromatogram of carotenoid extract of T. californicus fed with marigold powder rich in meso-zeaxanthin. Peak V is violaxanthin. (D) Spectra of astaxanthin peaks detected in the HPLC analysis.