Figure 11.
Evaluation of the protective efficacy of different treatments. Pigs in all groups were challenged by the highly virulent porcine epidemic diarrhea virus Pintung 52 (PEDV-PT) strain passage 7. The post-challenge pigs were monitored for stool consistency and fecal viral loads for 13 days. (A–C) The result of stool consistency scoring in each group. According to the stool consistency, the stool was graded as 0 for normal; 1 for loose feces; 2 for semi-fluid feces; and 3 for watery feces. The total number of pigs in the control, VLP, and VLP + CCL groups was 4, 5, and 5 pigs, respectively. The Arabic numerals labeled in the bar indicate the individuals in each group. (D) The result of fecal viral shedding in each group detected by probe-based quantitative reverse transcription PCR (RT-qPCR) targeting the PEDV N gene. The results were presented as mean value of log10 RNA copies/mL ± SEM. The detection limit of RT-qPCR was 4.7 log10 (copies/mL) marked as a dotted line.