Table 2.
Gene | Protein | Primer Sequence (5’-3’) |
---|---|---|
16S rRNA | 16S ribosomal protein | F: GTAGTACGATGGCGAAACTGC R: CTTCTCGACCCGAGGGACTT |
fliC | flagellum subunit | F: GCAGATGACGGTACATCCAA R: CCAGATCAGGCTGTGCTTTA |
hilA | SPI-1 transcriptional regulator | F: AATGGTCACAGGCTGAGGTG R: ACATCGTCGCGACTTGTGAA |
hilD | SPI-1 transcriptional regulator | F: CTCTGTGGGTACCGCCATTT R: TGCTTTCGGAGCGGTAAACT |
invH | adherence and invasion | F: GGTGCCCCTCCCTTCCT R: TGCGTTGGCCAGTTGCT |
prgH | T3SS needle support at membrane | F: TGAACGGCTGTGAGTTTCCA R: GCGCATCACTCTGACCTACCA |
prgK | T3SS needle support at membrane | F: GGGTGGAAATAGCGCAGATG R: TCAGCTCGCGGAGACGATA |
sipA | actin binding protein for cell invasion | F: CGTCTTCGCCTCAGGAGAAT R: TGCCGGGCTCTTTCGTT |