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. 2020 Oct 3;9(10):668. doi: 10.3390/antibiotics9100668

Table 2.

Primers used in determining gene expression of ST strains.

Gene Protein Primer Sequence (5’-3’)
16S rRNA 16S ribosomal protein F: GTAGTACGATGGCGAAACTGC
R: CTTCTCGACCCGAGGGACTT
fliC flagellum subunit F: GCAGATGACGGTACATCCAA
R: CCAGATCAGGCTGTGCTTTA
hilA SPI-1 transcriptional regulator F: AATGGTCACAGGCTGAGGTG
R: ACATCGTCGCGACTTGTGAA
hilD SPI-1 transcriptional regulator F: CTCTGTGGGTACCGCCATTT
R: TGCTTTCGGAGCGGTAAACT
invH adherence and invasion F: GGTGCCCCTCCCTTCCT
R: TGCGTTGGCCAGTTGCT
prgH T3SS needle support at membrane F: TGAACGGCTGTGAGTTTCCA
R: GCGCATCACTCTGACCTACCA
prgK T3SS needle support at membrane F: GGGTGGAAATAGCGCAGATG
R: TCAGCTCGCGGAGACGATA
sipA actin binding protein for cell invasion F: CGTCTTCGCCTCAGGAGAAT
R: TGCCGGGCTCTTTCGTT