Figure 1.

Change over time in repeated paclitaxel (PTX) treatment-induced cold (A) and mechanical (B) allodynia in mice. On days 0, 2, 4, and 6 (4 arrows), PTX or vehicle was administered (i.p.). Mice were subjected to measurement of allodynia just before and between day 4 to day 21 after the first dose of PTX (on the timeline: days 0, 4, 7, 10, 13, 16, 19, and 21, respectively). In the acetone test, the number of licking and shaking motions in 30 s were counted. In the von Frey assay, the percentage was used to express the result: (number of tests accompanied by withdrawals) × 100/(number of total tests). Data are expressed as mean ± S.E.M.; ** p < 0.01, *** p < 0.001, vs. vehicle by Bonferroni post-test after two-way analysis of variance (ANOVA).