Figure 1.

Fluctuations in total mean crystals area per leaf of C. quitensis plants under ambient or low CO₂ concentration. (a), Boxplots representing the effect of the CO₂ limitation treatment on leaf crystals area per leaf (%). The horizontal line indicates the mean and length of each whisker indicates the interquartile range (IQR); n = 14, different letters represent statistically significant differences (Two-way ANOVA; p < 0.05). In (a), plants were kept over time under constant light in airtight chambers injected either with ambient air (Control, 400 ppm CO₂, white boxes) or filtered air with soda lime (CO₂ limitation, 11.5 ppm CO₂, gray boxes). (b), Diurnal fluctuations in CaOx crystal area in C. quitensis leaves. Plants were grown under optimal growth conditions. Yellow background denotes light hours, while gray background dark hours. Error bars denote SE of mean; n = 15. Different letters represent statistically significant differences between time of the day (one-way ANOVA; p < 0.05).