Figure 2.
Production scheme of an anti-BCMA Fab immunotoxin. (A) Schematics showing expression plasmids encoding the heavy chain linked to toxin and the second encoding the light chain. Both plasmids contain an inducible T7 transcription promoter and a chloramphenicol antibiotic resistance gene (CMR) as selectable marker. (B) Schematics of correctly folded Fab immunotoxin protein. (C) Analytical PAGE gel showing the quality of purified Fab immunotoxin. Lanes: NR, protein analyzed under non-reducing condition; R, protein analyzed under reducing condition; M, molecular weight standards.