Nutrient Generality of Migration Rate Evolution
The 16 strains isolated after 10 rounds of selection (four from each nutrient condition, Figures 1B–1E) were assayed for enhanced migration rate in each of the four nutrient conditions used for the selection experiments: (A) mannose, (B) melibiose, (C) NAG and (D) galactose. Migration rates of evolved strains are presented as mean ± standard error of two replicate plates for each strain in each condition, except as noted below. Marker shapes denote selection condition; marker colors denote assay condition (legend). Migration rates of the founding strain (F) in each condition are presented as mean ± standard error of 3–4 replicate plates in each condition. For the following exceptions, error bars are standard error on migration rate from a single plate: The rightmost NAG-evolved strain and leftmost gal-evolved strain had only one usable plate in in NAG (C). For the rightmost two galactose-evolved strains in galactose (D), rates are reproduced from round 10 of the selection experiments (Figure 1E). See also Figures S2 and S3 and Table S1.